Molecular detection and characterization of Hop stunt viroid sequence variants from naturally infected pomegranate (Punica granatum L.) in Tunisia
Tunisian pomegranate Hop stunt viroid (HSVd) variants are described.Dot-blot hybridization, S-Page, and reverse transcription polymerase chain reaction Gas Range (RT-PCR) of RNA extracts from infected tissues were carried out.Results obtained by these techniques were confirmed by cDNA sequencing.The genetic diversity among the Tunisian variants was